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Apoptosis regulator BAX

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(Redirected from Bcl-2-associated X protein)

BAX
Available structures
PDBOrtholog search: PDBe RCSB
Identifiers
AliasesBAX, BCL2L4, BCL2 associated X protein, BCL2 associated X, apoptosis regulator
External IDsOMIM: 600040; MGI: 99702; HomoloGene: 7242; GeneCards: BAX; OMA:BAX - orthologs
Orthologs
SpeciesHumanMouse
Entrez

581

12028

Ensembl

ENSG00000087088

ENSMUSG00000003873

UniProt

Q07812
Q5ZPJ0

Q07813

RefSeq (mRNA)

NM_007527

RefSeq (protein)

NP_031553

Location (UCSC)Chr 19: 48.95 – 48.96 MbChr 7: 45.11 – 45.12 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

Apoptosis regulator BAX, also known as bcl-2-like protein 4, is a protein that in humans is encoded by the BAX gene.[5] BAX is a member of the Bcl-2 gene family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis.[6]

Structure

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The BAX gene was the first identified pro-apoptotic member of the Bcl-2 protein family.[7] Bcl-2 family members share one or more of the four characteristic domains of homology entitled the Bcl-2 homology (BH) domains (named BH1, BH2, BH3 and BH4), and can form hetero- or homodimers.[7][8] These domains are composed of nine α-helices, with a hydrophobic α-helix core surrounded by amphipathic helices and a transmembrane C-terminal α-helix anchored to the mitochondrial outer membrane (MOM). A hydrophobic groove formed along the C-terminal of α2 to the N-terminal of α5, and some residues from α8, binds the BH3 domain of other BAX or BCL-2 proteins in its active form. In the protein's inactive form, the groove binds its transmembrane domain, transitioning it from a membrane-bound to a cytosolic protein. A smaller hydrophobic groove formed by the α1 and α6 helices is located on the opposite side of the protein from the major groove, and may serve as a BAX activation site.[9]

Orthologs of the BAX gene have been identified in most mammals for which complete genome data are available.[10]

Function

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In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated.[11][12][13][14][15]

BAX is believed to interact with, and induce the opening of the mitochondrial voltage-dependent anion channel, VDAC.[16] Alternatively, growing evidence also suggests that activated BAX and/or Bak form an oligomeric pore, MAC in the MOM (mitochondrial outer membrane).[17][18] This results in the release of cytochrome c and other pro-apoptotic factors from the mitochondria, often referred to as mitochondrial outer membrane permeabilization, leading to activation of caspases.[19] This defines a direct role for BAX in mitochondrial outer membrane permeabilization. BAX activation is stimulated by various abiotic factors, including heat, hydrogen peroxide, low or high pH, and mitochondrial membrane remodeling. In addition, it can become activated by binding BCL-2, as well as non-BCL-2 proteins such as p53 and Bif-1. Conversely, BAX can become inactivated by interacting with VDAC2, Pin1, and IBRDC2.[9]

Clinical significance

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The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX. Wild-type p53 has been demonstrated to upregulate the transcription of a chimeric reporter plasmid utilizing the consensus promoter sequence of BAX approximately 50-fold over mutant p53. Thus it is likely that p53 promotes BAX's apoptotic faculties in vivo as a primary transcription factor. However, p53 also has a transcription-independent role in apoptosis. In particular, p53 interacts with BAX, promoting its activation as well as its insertion into the mitochondrial membrane.[20][21][22]

Drugs that activate BAX, such as ABT-737, a BH3 mimetic, hold promise as anticancer treatments by inducing apoptosis in cancer cells.[9] For instance, binding of HA-BAD to BCL-xL and concomitant disruption of BAX:BCL-xL interaction was found to partly reverse paclitaxel resistance in human ovarian cancer cells.[23] Meanwhile, excessive apoptosis in such conditions as ischemia reperfusion injury and amyotrophic lateral sclerosis may benefit from drug inhibitors of BAX.[9]

Interactions

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Overview of signal transduction pathways involved with apoptosis.

Bcl-2-associated X protein has been shown to interact with:

See also

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References

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  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000003873 – Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ "UniProt". www.uniprot.org. Retrieved 30 April 2023.
  6. ^ "Entrez Gene: BCL2-associated X protein".
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  22. ^ Miyashita T, Reed JC (January 1995). "Tumor suppressor p53 is a direct transcriptional activator of the human bax gene". Cell. 80 (2): 293–299. doi:10.1016/0092-8674(95)90412-3. PMID 7834749. S2CID 14495370.
  23. ^ a b Strobel T, Tai YT, Korsmeyer S, Cannistra SA (November 1998). "BAD partly reverses paclitaxel resistance in human ovarian cancer cells". Oncogene. 17 (19): 2419–2427. doi:10.1038/sj.onc.1202180. PMID 9824152. S2CID 44240363.
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