3-Amino-9-ethylcarbazole

3-Amino-9-ethylcarbazole
Names
	Preferred IUPAC name 9-Ethyl-9H-carbazol-3-amine
	Other names AEC; 9-Ethylcarbazol-3-amine
Identifiers
CAS Number	132-32-1 ;
3D model (JSmol)	Interactive image;
ChEBI	CHEBI:122149;
ChEMBL	ChEMBL327035;
ChemSpider	8269 ;
ECHA InfoCard	100.004.599
EC Number	205-057-7;
MeSH	3-amino-9-ethylcarbazole
PubChem CID	8588;
UNII	8Q2BG27JBU ;
UN number	2811
CompTox Dashboard (EPA)	DTXSID1030319 ;
	InChI InChI=1S/C14H14N2/c1-2-16-13-6-4-3-5-11(13)12-9-10(15)7-8-14(12)16/h3-9H,2,15H2,1H3 Key: OXEUETBFKVCRNP-UHFFFAOYSA-N;
	SMILES CCN1C2=C(C=C(C=C2)N)C3=CC=CC=C31;
Properties
Chemical formula	C14H14N2
Molar mass	210.280 g·mol−1
Hazards
	Occupational safety and health (OHS/OSH):
Main hazards	Acutely toxic, Health hazards
	GHS labelling:
Pictograms
Signal word	Danger
Hazard statements	H350
Precautionary statements	P201, P202, P281, P308+P313, P405, P501
	Except where otherwise noted, data are given for materials in their standard state (at 25 °C [77 °F], 100 kPa). (what is ?) Infobox references

3-Amino-9-ethylcarbazole (AEC) is a chemical compound commonly used as a chromogenic substrate in immunohistochemistry, specifically for visualizing sections stained with HRP-conjugated secondary antibodies.^[1] After the chromogenic oxidation reaction catalyzed by HRP, a red water-insoluble precipitate is formed in situ, visualizing the location of the antigen detected by the HRP-conjugated antibody. The resulting stained section can be destained by organic solvents in which the red precipitate is soluble.

References

^ Edward C., Stack; Chichung, Wang; Kristin A., Roman; Clifford C., Hoyt (2014). "Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis". Methods. 70 (1): 46–58. doi:10.1016/j.ymeth.2014.08.016. PMID 25242720.

[1] Edward C., Stack; Chichung, Wang; Kristin A., Roman; Clifford C., Hoyt (2014). "Multiplexed immunohistochemistry, imaging, and quantitation: A review, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis". Methods. 70 (1): 46–58. doi:10.1016/j.ymeth.2014.08.016. PMID 25242720.

[1]