The 1900–01 St Helens R.F.C. season was the club's sixth in the Northern Rugby Football Union, the 27th in their history. In their lowest ever finish in a rugby league competition, coming only a year after their most fruitful one, the club finished 13th in the Lancashire Senior Championship, having two points docked for breach of professional rules in the process.[1] In the Challenge Cup, the club were knocked out in the third round by Batley.[2]