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WNT4

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WNT4
Identifiers
AliasesWNT4, SERKAL, WNT-4, Wnt family member 4
External IDsOMIM: 603490; MGI: 98957; HomoloGene: 22529; GeneCards: WNT4; OMA:WNT4 - orthologs
Orthologs
SpeciesHumanMouse
Entrez
Ensembl
UniProt
RefSeq (mRNA)

NM_030761

NM_009523

RefSeq (protein)

NP_110388

NP_033549

Location (UCSC)Chr 1: 22.12 – 22.14 MbChr 4: 137 – 137.03 Mb
PubMed search[3][4]
Wikidata
View/Edit HumanView/Edit Mouse

WNT4 is a secreted protein that, in humans, is encoded by the WNT4 gene, found on chromosome 1.[5][6] It promotes female sex development and represses male sex development. Loss of function may have consequences, such as female to male sex reversal.

Function

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The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and embryogenesis.[5]

Pregnancy

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WNT4 is involved in many features of pregnancy as a downstream target of BMP2. For example, it regulates endometrial stromal cell proliferation, survival, and differentiation.[7] These processes are all necessary for the development of an embryo. Ablation in female mice results in subfertility, with defects in implantation and decidualization. For instance, there is a decrease in responsiveness to progesterone signaling. Furthermore, postnatal uterine differentiation is characterized by a reduction in gland numbers and the stratification of the luminal epithelium.[7]

Sexual development

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Early gonads

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Gonads arise from the thickening of coelomic epithelium, which at first appears as multiple cell layers. They later commit to sex determination, becoming either female or male under normal circumstances. Regardless of sex, though, WNT4 is needed for cell proliferation.[8] In mouse gonads, it has been detected only eleven days after fertilization. If deficient in XY mice, there is a delay in Sertoli cell differentiation. Moreover, there is delay in sex cord formation. These issues are usually compensated for at birth.[8]

WNT4 also interacts with RSPO1 early in development. If both are deficient in XY mice, the outcome is less expression of SRY and downstream targets.[8] Furthermore, the amount of SOX9 is reduced and defects in vascularization are found. These occurrences result in testicular hypoplasia. Male to female sex reversal, however, does not occur because Leydig cells remain normal. They are maintained by steroidogenic cells, now unrepressed.[8]

Female Sexual Development

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Wnt4, is a growth factor and member of the Wnt gene family[9][10] that acts through frizzled receptors and intracellular signals which lead to transcriptional activation of a host of genes.[11] Wnt4 is involved in various developmental processes however, it is understood for its role in the development of the kidneys as well as in the development of the female reproductive tract and female secondary sex characteristics.[9] Wnt4 is expressed in the developing kidney, the mesonephros and the mesenchyme of the bipotential gonad,[9][12][13] and aids in development of the female reproductive tract. Specifically, by supporting oocyte development and regulating the formation of the mullerian duct, which will give rise to the oviduct, uterus, cervix and upper vagina. The growth factor also regulates steroidogenesis through upregulating genes such as Dax1, a gene expressed in the developing ovary and responsible for the inhibition of steroidogenic enzymes and ultimately the prevention of testis formation.[10][14][15] Models utilizing knockout mice have shown that the absence of Wnt4 results in the presence of steroidogenic enzymes, masculinization of female genitalia, failure of the wolffian duct to regress, absence of the mullerian duct as well as a decrease in oocyte numbers.[13] Studies utilizing the knockout mouse model have highlighted the importance of Wnt4 in female reproductive development.

Ovaries

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WNT4 is required for female sex development. Upon secretion it binds to Frizzled receptors, activating a number of molecular pathways. One important example is the stabilization of β catenin, which increases the expression of target genes.[16] For instance, TAFIIs 105 is now encoded, a subunit of the TATA binding protein for RNA polymerase in ovarian follicle cells. Without it, female mice have small ovaries with less mature follicles. In addition, the production of SOX9 is blocked.[17] In humans, WNT4 also suppresses 5-α reductase activity, which converts testosterone into dihydrotestosterone. External male genitalia are therefore not formed. Moreover, it contributes to the formation of the Müllerian duct, a precursor to female reproductive organs.[16]

Male sexual development

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The absence of WNT4 is required for male sex development. FGF signaling suppresses WNT4, acting in a feed forward loop triggered by SOX9. If this signaling is deficient in XY mice, female genes are unrepressed.[18] With no FGFR2, there is a partial sex reversal. With no FGF9, there is a full sex reversal. Both cases are rescued, though, by a WNT4 deletion. In these double mutants, the resulting somatic cells are normal.[18]

Kidneys

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WNT4 is essential for nephrogenesis. It regulates kidney tubule induction and the mesenchymal to epithelial transformation in the cortical region. In addition, it influences the fate of the medullary stroma during development. Without it, smooth muscle α actin is markedly reduced. This occurrence causes pericyte deficiency around the vessels, leading to a defect in maturation. WNT4 probably functions by activating BMP4, a known smooth muscle differentiation factor.[19]

Muscles

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WNT4 contributes to the formation of the neuromuscular junction in vertebrates. Expression is high during the creation of first synaptic contacts, but subsequently downregulated.[20] Moreover, loss of function causes a 35 percent decrease in the number of acetylcholine receptors. Overexpression, however, causes an increase. These events alter fiber type composition with the production of more slow fibers. Lastly, MuSK is the receptor for WNT4, activated through tyrosine phosphorylation. It contains a CRD domain similar to Frizzled receptors.[20]

Lungs

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WNT4 is also associated with lung formation and has a role in the formation of the respiratory system. When WNT4 is knocked out, there are many problems that occur in lung development. It has been shown that when WNT4 is knocked out, the lung buds formed are reduced in size and proliferation has greatly diminished which cause underdeveloped or incomplete development of the lungs. It also causes tracheal abnormalities because it affects the tracheal cartilage ring formation. Lastly, the absence of WNT4 also affects the expression of other genes that function in lung development such as Sox9 and FGF9.[21]

Clinical significance

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Deficiency

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Several mutations are known to cause loss of function in WNT4. One example is a heterozygous C to T transition in exon 2.[22] This causes an arginine to cysteine substitution at amino acid position 83, a conserved location. The formation of illegitimate sulfide bonds creates a misfolded protein, resulting in loss of function. In XX humans, WNT4 now cannot stabilize β-catenin.[22] Furthermore, steroidogenic enzymes like CYP17A1 and HSD3B2 are not suppressed, leading to an increase in testosterone production. Along with this androgen excess, patients have no uteruses. Other Müllerian abnormalities, however, are not found. This disorder is therefore distinct from classic Mayer-Rokitansky-Kuster-Hauser syndrome.[22]

SERKAL syndrome

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A disruption of WNT4 synthesis in XX humans produces SERKAL syndrome. The genetic mutation is a homozygous C to T transition at cDNA position 341.[16] This causes an alanine to valine residue substitution at amino acid position 114, a location highly conserved in all organisms, including zebrafish and Drosophila. The result is loss of function, which affects mRNA stability. Ultimately it causes female to male sex reversal.[16]

Mayer-Rokitansky-Kuster-Hauser Syndrome

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WNT4 has been clearly implicated in the atypical version of Mayer-Rokitansky-Kuster-Hauser Syndrome found in XX humans. A genetic mutation causes a leucine to proline residue substitution at amino acid position 12.[23] This occurrence reduces the intranuclear levels of β-catenin. In addition, it removes the inhibition of steroidogenic enzymes like 3β-hydroxysteriod dehydrogenase and 17α-hydroxylase. Patients usually have uterine hypoplasia, which is associated with biological symptoms of androgen excess. Furthermore, Müllerian abnormalities are often found.[23]

References

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  1. ^ a b c GRCh38: Ensembl release 89: ENSG00000162552Ensembl, May 2017
  2. ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000036856Ensembl, May 2017
  3. ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  4. ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
  5. ^ a b "Entrez Gene: wingless-type MMTV integration site family".
  6. ^ Huguet EL, McMahon JA, McMahon AP, Bicknell R, Harris AL (May 1994). "Differential expression of human Wnt genes 2, 3, 4, and 7B in human breast cell lines and normal and disease states of human breast tissue". Cancer Research. 54 (10): 2615–21. PMID 8168088.
  7. ^ a b Franco HL, Dai D, Lee KY, Rubel CA, Roop D, Boerboom D, Jeong JW, Lydon JP, Bagchi IC, Bagchi MK, DeMayo FJ (April 2011). "WNT4 is a key regulator of normal postnatal uterine development and progesterone signaling during embryo implantation and decidualization in the mouse". FASEB Journal. 25 (4): 1176–87. doi:10.1096/fj.10-175349. PMC 3058697. PMID 21163860.
  8. ^ a b c d Chassot AA, Bradford ST, Auguste A, Gregoire EP, Pailhoux E, de Rooij DG, Schedl A, Chaboissier MC (December 2012). "WNT4 and RSPO1 together are required for cell proliferation in the early mouse gonad". Development. 139 (23): 4461–72. doi:10.1242/dev.078972. PMID 23095882.
  9. ^ a b c Bernard P, Harley VR (January 2007). "Wnt4 action in gonadal development and sex determination". The International Journal of Biochemistry & Cell Biology. 39 (1): 31–43. doi:10.1016/j.biocel.2006.06.007.
  10. ^ a b Biason-Lauber A, Konrad D (2008). "WNT4 and Sex Development". Sexual Development. 2 (4–5): 210–218. doi:10.1159/000152037.
  11. ^ Dale CT (15 January 1998). "Signal transduction by the Wnt family of ligands". Biochemical Journal. 329 (2): 209–223. doi:10.1042/bj3290209. PMC 1219034.
  12. ^ Stark K, Vainio S, Vassileva G, McMahon AP (December 1994). "Epithelial transformation of metanephric mesenchyme in the developing kidney regulated by Wnt-4". Nature. 372 (6507): 679–683. doi:10.1038/372679a0.
  13. ^ a b Vainio S, Heikkilä M, Kispert A, Chin N, McMahon AP (February 1999). "Female development in mammals is regulated by Wnt-4 signalling". Nature. 397 (6718): 405–409. doi:10.1038/17068.
  14. ^ Goodfellow PN, Camerino G (June 1999). "DAX-1, an 'antitestis'gene:". Cellular and Molecular Life Sciences. 55 (6–7): 857–863. doi:10.1007/pl00013201. PMC 11147076.
  15. ^ Jordan BK, Mohammed M, Ching ST, Délot E, Chen XN, Dewing P, Swain A, Rao PN, Elejalde BR, Vilain E (May 2001). "Up-Regulation of WNT-4 Signaling and Dosage-Sensitive Sex Reversal in Humans". The American Journal of Human Genetics. 68 (5): 1102–1109. doi:10.1086/320125. PMC 1226091.
  16. ^ a b c d Mandel H, Shemer R, Borochowitz ZU, Okopnik M, Knopf C, Indelman M, Drugan A, Tiosano D, Gershoni-Baruch R, Choder M, Sprecher E (January 2008). "SERKAL syndrome: an autosomal-recessive disorder caused by a loss-of-function mutation in WNT4". American Journal of Human Genetics. 82 (1): 39–47. doi:10.1016/j.ajhg.2007.08.005. PMC 2253972. PMID 18179883.
  17. ^ Gilbert S (2010). Developmental Biology (9th ed.). Massachusetts: Sinauer Associates.
  18. ^ a b Jameson SA, Lin YT, Capel B (October 2012). "Testis development requires the repression of Wnt4 by Fgf signaling". Developmental Biology. 370 (1): 24–32. doi:10.1016/j.ydbio.2012.06.009. PMC 3634333. PMID 22705479.
  19. ^ Itäranta P, Chi L, Seppänen T, Niku M, Tuukkanen J, Peltoketo H, Vainio S (May 2006). "Wnt-4 signaling is involved in the control of smooth muscle cell fate via Bmp-4 in the medullary stroma of the developing kidney". Developmental Biology. 293 (2): 473–83. doi:10.1016/j.ydbio.2006.02.019. PMID 16546160.
  20. ^ a b Strochlic L, Falk J, Goillot E, Sigoillot S, Bourgeois F, Delers P, Rouvière J, Swain A, Castellani V, Schaeffer L, Legay C (2012). "Wnt4 participates in the formation of vertebrate neuromuscular junction". PLOS ONE. 7 (1): e29976. Bibcode:2012PLoSO...729976S. doi:10.1371/journal.pone.0029976. PMC 3257248. PMID 22253844.
  21. ^ Caprioli A, Villasenor A, Wylie LA, Braitsch C, Marty-Santos L, Barry D, Karner CM, Fu S, Meadows SM, Carroll TJ, Cleaver O (October 2015). "Wnt4 is essential to normal mammalian lung development". Developmental Biology. 406 (2): 222–34. doi:10.1016/j.ydbio.2015.08.017. PMC 7050435. PMID 26321050.
  22. ^ a b c Biason-Lauber A, De Filippo G, Konrad D, Scarano G, Nazzaro A, Schoenle EJ (January 2007). "WNT4 deficiency--a clinical phenotype distinct from the classic Mayer-Rokitansky-Kuster-Hauser syndrome: a case report". Human Reproduction. 22 (1): 224–9. doi:10.1093/humrep/del360. PMID 16959810.
  23. ^ a b Sultan C, Biason-Lauber A, Philibert P (January 2009). "Mayer-Rokitansky-Kuster-Hauser syndrome: recent clinical and genetic findings". Gynecological Endocrinology. 25 (1): 8–11. doi:10.1080/09513590802288291. PMID 19165657. S2CID 33461252.

Further reading

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This article incorporates text from the United States National Library of Medicine, which is in the public domain.