Talk:Polarized light microscopy

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Leaving these images here in case editors of this page find them useful. Uploaded by the creator for the Wiki Science Competition (the group was one of the finalists in its category). Captions are the uploader's notes, which look to be intended to be taken together. — Rhododendrites ^talk \\ 06:20, 26 January 2018 (UTC)[reply]

the thickness of the crystal and the concentration of the original solution can produce different colors
melting the original vitamin c crystals and allowing them to recrystalize changes the appearance again
material (in this case collagen fibers) appear bright or dark depending on their orientation with respect to the polarizing filters - hence, tissue organization can be quantified.
Similarly, this is an image of haematoxylin used to stain the wavy collagen.
as the thickness of collagen fibers in scar tissue increases, their color changes from green to yellow to orange
The dyes used to stain tissue in histology often form crystals often form crystals. This is the dye (sirius red) used to stain the scar collagen in one of the previous images.
polarized light can also be used the assess tissue architecture - here, we see the striated bands of cardiac muscle (green)