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File:Unchained Ligation Sequencing.png

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Summary

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English: 1. Single strand of DNA from a DNA nanoball 2. Attach complementary anchor to known adapter sequence 3. Add probe set #1 and DNA Ligase to interrogate position #1 to the left of the anchor. Only the complementary probe binds 4. Wash away all unbound probes and Ligase, and read the color of the fluorophore 5. Denature anchor and probe from DNA nanoball and add another anchor 6. Add probe set #2 and DNA ligase to interrogate position #2 to the left of the anchor. Repeat.
Source Own work
Author Suspencewl

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Date/TimeThumbnailDimensionsUserComment
current20:14, 28 February 2011Thumbnail for version as of 20:14, 28 February 2011902 × 1,026 (457 KB)SuspencewlRemoved black side bars
02:06, 25 February 2011Thumbnail for version as of 02:06, 25 February 20111,282 × 1,026 (154 KB)Suspencewl{{Information |Description ={{en|1=1. Single strand of DNA from a DNA nanoball 2. Attach complementary anchor to known adapter sequence 3. Add probe set #1 and DNA Ligase to interrogate position #1 to the left of the anchor. Only the complementary pro

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