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Description
English: Generic diagram of Sleeping Beauty transposase-mediated cut-and-paste transposition. Figure 1. Mechanism of SB-mediated transposition. Top line: A transposon, defined by the mirrored sets of red double arrows (IR/DRs) is shown as contained in another DNA molecule (e.g., a plasmid shown by the blue lines). The transposon in this example harbors an expression cassette consisting of a promoter (blue oval) that can direct transcription of the gene or other DNA sequence labeled “genetic cargo”. Middle lines: Sleeping Beauty (SB) transposase binds to the IR/DRs as shown and cuts the transposon out of the plasmid (the cut sites are indicated by the two black slashed lines in the remaining plasmid) Bottom two lines: Another DNA molecule (green) with a TA sequence can become the recipient of a transposed transposon. In the process, the TA sequence at the insertion site is duplicated.
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Daniel Zerull

Perryhackett
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9 May 2011

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Date/TimeThumbnailDimensionsUserComment
current19:17, 4 March 2019Thumbnail for version as of 19:17, 4 March 2019720 × 424 (51 KB)Artoria2e5Crop away built-in caption. Textual version now in description.
21:27, 9 May 2011Thumbnail for version as of 21:27, 9 May 2011720 × 540 (97 KB)Perryhackett
21:24, 9 May 2011Thumbnail for version as of 21:24, 9 May 2011720 × 540 (97 KB)Perryhackett{{Information |Description ={{en|1=Generic diagram of Sleeping Beauty transposase-mediated cut-and-paste transposition.}} |Source ={{own}} |Author =Perryhackett |Date =2011-05-09 |Permission =not need

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