File:Onion flake. Cells. SEM-BSE.jpg
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Summary
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Русский: Клетки внутренней поверхности чешуйки лука. Под шагреневой поверхностью клеточной стенки, в толще цитоплазмы, видно ядро и мелкие органеллы. Изображение в обратно-рассеянных электронах (BSE), полученное после контрастирования лантаноидами (BioREE, Россия) на сканирующем электронном микроскопе EVO LS10 (Zeiss, Германия) в режиме низкого вакуума без фиксации, обезвоживания и напыления образца. Такая схема работы с биологическими объектами, разработанная в лаборатории фундаментальных исследований ФГБНУ «НИИ глазных болезней», делает клетки «полупрозрачными» для электронного пучка и позволяет визуализировать на электронном микроскопе их подповерхностный слой, приближая сканирующую электронную микроскопию к оптической. Рассматривание подповерхностного слоя клеток на сканирующем электронном микроскопе при таком способе визуализации очень похоже на рассматривание пузырьков в толще льда на поверхности замерзшего пруда — чем объекты глубже, тем они менее контрастные и более размытые. Соавтор: Суббот А.М. English: Epidermal cells from the inner surface of an onion flake. Beneath the shagreen-like cell walls one can see nuclei and small organelles floating in the cytoplasm. This BSE-image of a lanthanoid-stained sample was taken with EVO LS10 scanning electron microscope (Zeiss, Germany) without prior fixation, nor dehydration, nor sputtering. The unique method of sample preparation used here (BioREE, Russia, with support from the Research Institute of Eye Diseases, Russia) provides an outstanding contrast between subcellular structures giving an impression of light microscopy but at the resolution of SEM. A sample thus prepared resembles a block of ice with air bubbles in it – the deeper an object lies, the lower contrast it has and the more diffused it appears. Coauthor: A.M. Subbot |
| Date | |
| Source | Own work |
| Author | Ivan A. Novikov |
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This image was uploaded as part of European Science Photo Competition 2015. |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 11:55, 11 November 2015 | | 3,072 × 2,304 (3.43 MB) | Novikov76 | User created page with UploadWizard |
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