File:Multiplexed mapping of mRNA expression within an intact zebrafish embryo.png
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Summary
| Description |
English: Simultaneous mapping of five different target mRNAs within an intact zebrafish embryo using scRNA signal amplification and confocal microscopy. RNA probes complementary to mRNA targets trigger chain reactions in which fluorophore- labeled scRNAs self-assemble into tethered fluorescent amplification polymers. The programmability and sequence selectivity of these amplification cascades enable five scRNA amplifiers to operate independently at the same time in the same sample, each staining for expression of one of the five target mRNAs. Scale bar: 50 μm. Image from Choi et al. 2010; http://www.nature.com/nbt/journal/v28/n11/full/nbt.1692.html; used with permission of the Nature Publishing Group. |
| Date | |
| Source | http://www.nature.com/nbt/journal/v28/n11/full/nbt.1692.html |
| Author | H.M.T. Choi, J.Y. Chang, L.A. Trinh, J.E. Padilla, S.E. Fraser, and N.A. Pierce |
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| current | 20:46, 18 February 2015 | | 1,033 × 636 (598 KB) | EditorMAK | User created page with UploadWizard |
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