File:Kalda-kammjalg (Sida crystallina).jpg
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Size of this preview: 397 × 599 pixels. Other resolutions: 159 × 240 pixels | 318 × 480 pixels | 509 × 768 pixels | 679 × 1,024 pixels | 1,357 × 2,048 pixels | 3,410 × 5,144 pixels.
Original file (3,410 × 5,144 pixels, file size: 7.35 MB, MIME type: image/jpeg)
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Summary
DescriptionKalda-kammjalg (Sida crystallina).jpg |
Eesti: Kalda-kammjalg (Sida crystallina) pildistatud mikroskoobi all kasutades 8x objektiivi ja tumevälja mikroskoopiat. Isend on kogutud Lääne-Virumaal asuvast Neeruti järvest 2022. aasta juunis. See on üks suuremaid ja levinumaid litoraali vesikirpe meie veekogudes. Pildil on hästi näha liigile iseloomulik ristkülikukujuline läbipaistev keha ning suur ventraalselt paiknev liitsilm, mis tumevälja valgustuses eriti silmatorkavalt oranžilt helendab. Looma seljal olevas haudetaskus on näha kaks arenevat järglast, keda on võimalik tuvastada nende tumedate silmade järgi. Läbi läbipaistva kesta on näha ka kollakasrohelisi struktuure.
Pilt on tehtud kasutades tumevälja mikroskoopiat, kus valguskiired suunatakse objektile külgedelt spetsiaalse kondensoriga nii, et otse objektiivi ei jõua ükski kiir. Mikroskoobis näeme ainult neid valguskiiri, mis peegelduvad või hajuvad objektilt. See tehnika toob eriti hästi esile läbipaistvate objektide piirjooned ja sisemised struktuurid, luues tumeda tausta peal helendava kujutise. Pilt on tehtud kasutades fookuse virnastamise tehnikat (focus stacking), mis tagab suure teravussügavuse.
English: A crystal water flea (Sida crystallina) photographed under a microscope using an 8x objective and dark-field microscopy. The specimen was collected from Lake Neeruti in Lääne-Viru County, Estonia, in June 2022. This is one of the largest and most common littoral cladocerans in our water bodies. The image clearly shows the species' characteristic rectangular transparent body and large ventrally positioned compound eye, which glows distinctively orange under dark-field illumination. Two developing embryos can be seen in the dorsal brood pouch, identifiable by their dark eyes. Through the transparent carapace, yellowish-green structures are also visible.
The image was captured using dark-field microscopy, where light rays are directed at the specimen from the sides using a special condenser so that no direct light reaches the objective lens. In the microscope, we only see the light that is reflected or scattered by the specimen. This technique particularly emphasizes the contours and internal structures of transparent objects, creating a bright image against a dark background. The image was created using focus stacking technique, which ensures great depth of field throughout the subject. |
Date | |
Source | Own work |
Author | Janek Lass |
Licensing
I, the copyright holder of this work, hereby publish it under the following license:
This file is licensed under the Creative Commons Attribution 4.0 International license.
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This image was uploaded as part of Estonian science photo campaign.
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Selles failis kujutatud üksused
depicts
Teatud väärtus ilma Vikiandmete üksuseta
11 June 2022
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Date/Time | Thumbnail | Dimensions | User | Comment | |
---|---|---|---|---|---|
current | 14:53, 17 November 2024 | 3,410 × 5,144 (7.35 MB) | Janeklass | Uploaded own work with UploadWizard |
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Metadata
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Camera manufacturer | Canon |
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Camera model | Canon EOS 650D |
Exposure time | 1/4 sec (0.25) |
ISO speed rating | 100 |
Date and time of data generation | 11:02, 11 June 2022 |
User comments | Stacked from 100 images. Method=B (R=8,S=4) |
Width | 3,410 px |
Height | 5,144 px |
Bits per component |
|
Pixel composition | RGB |
Orientation | Normal |
Number of components | 3 |
Horizontal resolution | 300 dpi |
Vertical resolution | 300 dpi |
Software used | Adobe Photoshop 23.3 (Windows) |
File change date and time | 11:25, 11 June 2022 |
Exposure Program | Aperture priority |
Exif version | 2.31 |
Date and time of digitizing | 11:02, 11 June 2022 |
Shutter speed | 2 |
Exposure bias | −1.3333333333333 |
Metering mode | Spot |
Flash | Flash did not fire, compulsory flash suppression |
DateTimeOriginal subseconds | 88 |
DateTimeDigitized subseconds | 88 |
Color space | sRGB |
Exposure mode | Auto exposure |
White balance | Auto white balance |
Scene capture type | Standard |
Serial number of camera | 053053054593 |
Date metadata was last modified | 14:25, 11 June 2022 |
Unique ID of original document | D3D6EE2CA3013057F4A36CBE3399B078 |