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File:Kalda-kammjalg (Sida crystallina).jpg

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Eesti: Kalda-kammjalg (Sida crystallina) pildistatud mikroskoobi all kasutades 8x objektiivi ja tumevälja mikroskoopiat. Isend on kogutud Lääne-Virumaal asuvast Neeruti järvest 2022. aasta juunis. See on üks suuremaid ja levinumaid litoraali vesikirpe meie veekogudes. Pildil on hästi näha liigile iseloomulik ristkülikukujuline läbipaistev keha ning suur ventraalselt paiknev liitsilm, mis tumevälja valgustuses eriti silmatorkavalt oranžilt helendab. Looma seljal olevas haudetaskus on näha kaks arenevat järglast, keda on võimalik tuvastada nende tumedate silmade järgi. Läbi läbipaistva kesta on näha ka kollakasrohelisi struktuure. Pilt on tehtud kasutades tumevälja mikroskoopiat, kus valguskiired suunatakse objektile külgedelt spetsiaalse kondensoriga nii, et otse objektiivi ei jõua ükski kiir. Mikroskoobis näeme ainult neid valguskiiri, mis peegelduvad või hajuvad objektilt. See tehnika toob eriti hästi esile läbipaistvate objektide piirjooned ja sisemised struktuurid, luues tumeda tausta peal helendava kujutise. Pilt on tehtud kasutades fookuse virnastamise tehnikat (focus stacking), mis tagab suure teravussügavuse.
English: A crystal water flea (Sida crystallina) photographed under a microscope using an 8x objective and dark-field microscopy. The specimen was collected from Lake Neeruti in Lääne-Viru County, Estonia, in June 2022. This is one of the largest and most common littoral cladocerans in our water bodies. The image clearly shows the species' characteristic rectangular transparent body and large ventrally positioned compound eye, which glows distinctively orange under dark-field illumination. Two developing embryos can be seen in the dorsal brood pouch, identifiable by their dark eyes. Through the transparent carapace, yellowish-green structures are also visible. The image was captured using dark-field microscopy, where light rays are directed at the specimen from the sides using a special condenser so that no direct light reaches the objective lens. In the microscope, we only see the light that is reflected or scattered by the specimen. This technique particularly emphasizes the contours and internal structures of transparent objects, creating a bright image against a dark background. The image was created using focus stacking technique, which ensures great depth of field throughout the subject.
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Author Janek Lass

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Captions

A crystal water flea (Sida crystallina) photographed in lateral view using dark-field microscopy, showing characteristic rectangular transparent body and large compound eye.

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11 June 2022

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current14:53, 17 November 2024Thumbnail for version as of 14:53, 17 November 20243,410 × 5,144 (7.35 MB)JaneklassUploaded own work with UploadWizard

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