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Summary
| Description |
English: A pipeline for the production of anti-sRNA ASOs to reprogram gene expression. The input is an sRNA–mRNA interaction of interest. Anti-sRNA ASOs are rationally designed to target the sRNA. An in vitro sRNA–mRNA interaction assay is established. The anti-sRNA ASOs are screened in the in vitro sRNA–mRNA interaction assay. Promising anti-sRNA ASOs are evaluated in a gene expression assay. The output anti-sRNA ASO gene expression modulator can be taken forward for future applications, e.g., integration into synthetic gene circuits or the development of antibacterial ASOs. |
| Date | |
| Source | https://doi.org/10.1021/acssynbio.0c00603 |
| Author | Charlotte A. Henderson, Helen A. Vincent, and Anastasia J. Callaghan |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 00:41, 23 October 2022 | | 1,753 × 353 (111 KB) | Redheadedoctopus | Uploaded a work by Charlotte A. Henderson, Helen A. Vincent, and Anastasia J. Callaghan from https://doi.org/10.1021/acssynbio.0c00603 with UploadWizard |
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