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DescriptionHis-tag.png
English: Adding polyhistidine tags. A) The His-tag is added by inserting the DNA coding a protein of interest in a vector that has the tag ready to fuse at the C-terminal. B)The His-tag is added using primers containing the tag, after a PCR reaction the tag gets fused to the N-terminal of the gene.
AAAsBiophysics 20:29, 17 July 2007 (UTC)
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2007-07-17 19:18 Jfhaller 773×607× (31177 bytes) Adding polyhistidine tags. A) The His-tag is added by inserting the DNA coding a protein of interest in a vector that has the tag ready to fuse at the C-terminal. B)The His-tag is added using primers containing the tag, after a PCR reaction the tag gets f
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