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File:HCR-FISH visualization of collagen expression in P. waltl.jpg

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English: Top: Black and white bright-field microscopic view of four salamanders (Pleurodeles waltl) at developmental stages 35-37. Specimens 1 and 2 are captured ventrally, whereas specimens 3 and 4 are imaged dorsally. Among these, specimens 1, 3, and 4 are leucistic mutants, characterized by a knocked-out pigmentation gene, while specimen 2 is a wild-type, exhibiting no genetic mutations.

Bottom: The bottom panel illustrates the collagen expression patterns (CO2A1 gene) in these same salamanders, employing Hybridization Chain Reaction RNA Fluorescence In Situ Hybridization (HCR RNA-FISH) technology. The fluorescent visualization predominantly highlights the skeletal areas where collagen is abundantly present. Nonetheless, collagen expression is also observable in other regions, including the gills and on a distinct patch on top of the head.

The image was captured by Lennart Rikk from the Regenerative Immunology Lab (https://leighnd.github.io/) using Leica M205 FCA microscope.
Eesti: Üleval: Mustvalge valgusmikroskoobi pilt neljast salamandrist (Pleurodeles waltl) arengufaasides 35-37. Isendid 1 ja 2 on pildil ventraalselt, isendid 3 ja 4 dorsaalselt. Isendid 1, 3 ja 4 leukistilised mutandid, mida iseloomustab välja lülitatud pigmendigeen, samas kui isend 2 on metsiktüüpi, ilma geneetiliste mutatsioonideta.

All: Visualiseeritud on kollageeni ekspressioon (CO2A1 geen) samades salamandrites kasutades hübridisatsiooni ahelreaktsiooni RNA fluorestsentsi in situ hübridisatsiooni (HCR RNA-FISH) tehnoloogiat. Fluorestsents tõstab esile peamiselt luustiku piirkonna, kus kollageeni on rohkelt. Siiski on kollageeni ekspressioon märgatav ka teistes piirkondades nagu lõpustes ja pealeal eristuval laigul.

Pildi tegi Lennart Rikk Regeneratiivse Immunoloogia Laborist (https://leighnd.github.io/) mikroskoobiga Leica M205 FCA.
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Author Yodalr

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Analysis of collagen expression in young Salamanders (P. waltl) via HCR FISH visualization

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Iberian ribbed newt

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15 December 2023

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