File:FISSEQ schematic.png
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Summary
| Description |
English: FISSEQ schematic. A tagged random hexamer primer is used to prime M-MuLV reverse transcriptase to generate aminoallyl dUTP- modified cDNA fragments in fixed cells or tissues. BS(PEG)9 permanently cross-links the modified cDNA and the cellular protein matrix. After cDNA circularization, Phi29 DNA polymerase generates cDNA amplicon. The cross-linked amplicons in the 3D in situ RNA within the cell is sequenced. |
| Date | |
| Source | Own work |
| Author | Sinverso |
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| Date/Time | Thumbnail | Dimensions | User | Comment | |
|---|---|---|---|---|---|
| current | 04:35, 23 January 2016 | | 3,046 × 6,804 (566 KB) | Sinverso | Cross-wiki upload from en.wikipedia.org |
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