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Summary

Description A sandwich ELISA. (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked secondary antibody is added, and binds to detecting antibody; (5) substrate is added, and is converted by enzyme to detectable form.
Date Designed 2006-05-08
Source Own work
Author Jeffrey M. Vinocur
Permission
(Reusing this file)		 Own work, attribution required (Multi-license with GFDL and Creative Commons CC-BY 2.5)
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Licensing

Jeffrey M. Vinocur, the copyright holder of this work, hereby publishes it under the following licenses:
GNU head Permission is granted to copy, distribute and/or modify this document under the terms of the GNU Free Documentation License, Version 1.2 or any later version published by the Free Software Foundation; with no Invariant Sections, no Front-Cover Texts, and no Back-Cover Texts. A copy of the license is included in the section entitled GNU Free Documentation License.
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attribution share alike 		This file is licensed under the Creative Commons Attribution-Share Alike 3.0 Unported license.
Attribution: Jeffrey M. Vinocur
You are free:
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  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
  • share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.
This licensing tag was added to this file as part of the GFDL licensing update.
w:en:Creative Commons
attribution
This file is licensed under the Creative Commons Attribution 2.5 Generic license.
Attribution: Jeffrey M. Vinocur
You are free:
  • to share – to copy, distribute and transmit the work
  • to remix – to adapt the work
Under the following conditions:
  • attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
You may select the license of your choice.

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Date/TimeThumbnailDimensionsUserComment
current21:44, 19 July 2013Thumbnail for version as of 21:44, 19 July 2013680 × 290 (24 KB)Prolineservercorrected binding positions of molecules
15:39, 9 May 2006Thumbnail for version as of 15:39, 9 May 2006640 × 320 (24 KB)JVinocur{{Information| |Description='''A sandwich ''ELISA'''''''. (1) Plate is coated with a capture antibody; (2) sample is added, and any antigen present binds to capture antibody; (3) detecting antibody is added, and binds to antigen; (4) enzyme-linked second

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