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File:Cassette mutagenesis.svg

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Summary

Description
English: In cassette mutagenesis, a mutagenized double-stranded DNA fragment is used to replace a fragment of the gene of interest, resulting in a mutated copy of that gene. This diagram illustrates this process using Golden Gate Assembly, but other cloning methods such as Gibson Assembly and standard restriction/ligation cloning can be used as well.
Date
Source Own work
Author Rob Hurt

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Captions

Cassette mutagenesis via Golden Gate Assembly

3 June 2022

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f58db7e2c6ef154043421bb5b4ca4810dc1fbd80

151,963 byte

784 pixel

512 pixel

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Date/TimeThumbnailDimensionsUserComment
current23:34, 3 June 2022Thumbnail for version as of 23:34, 3 June 2022512 × 784 (148 KB)Rob Hurtfixed fonts
23:23, 3 June 2022Thumbnail for version as of 23:23, 3 June 2022512 × 784 (117 KB)Rob HurtUploaded own work with UploadWizard

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