English: In cassette mutagenesis, a mutagenized double-stranded DNA fragment is used to replace a fragment of the gene of interest, resulting in a mutated copy of that gene. This diagram illustrates this process using Golden Gate Assembly, but other cloning methods such as Gibson Assembly and standard restriction/ligation cloning can be used as well.
to share – to copy, distribute and transmit the work
to remix – to adapt the work
Under the following conditions:
attribution – You must give appropriate credit, provide a link to the license, and indicate if changes were made. You may do so in any reasonable manner, but not in any way that suggests the licensor endorses you or your use.
share alike – If you remix, transform, or build upon the material, you must distribute your contributions under the same or compatible license as the original.