C8orf58
| C8orf58 | |||||||||||||||||||||||||||||||||||||||||||||||||||
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| Aliases | C8orf58, chromosome 8 open reading frame 58 | ||||||||||||||||||||||||||||||||||||||||||||||||||
| External IDs | MGI: 2145726; HomoloGene: 19540; GeneCards: C8orf58; OMA:C8orf58 - orthologs | ||||||||||||||||||||||||||||||||||||||||||||||||||
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Chromosome 8 open reading frame 58 is an uncharacterised protein that in humans is encoded by the C8orf58 gene.[5] The protein is predicted to be localized in the nucleus.
Gene
[edit]The C8orf58 gene is located on chromosome 8 at position 8p21.3. It spans a total of 4,550 base pairs and has seven exons. C8orf58 is flanked by the genes PDLIM2 and CCAR2.[6] There are no aliases. It is defined as a protein coding gene.[7]
mRNA
[edit]C8orf58 produces three transcript splice variants. The transcript of variant 1 represents the longest transcript and encodes the largest protein. It is 2,062 base pairs and contains seven exons. There are two other splice variants, produced by alternative splice sites.[8]
| Isoform | Exons | Length (base pairs) | Features |
|---|---|---|---|
| Transcript Variant 1 | 1, 2, 3, 4, 5, 6, 7 | 2062 | One upstream in-frame stop codon. |
| Transcript Variant 2 | 1, 2, 3, 4, 5, 6, 7 | 2038 | Alternate in-frame splice site in the 3' coding region. |
| Transcript Variant 3 | 1, 2, 3, 4, 5, 6 | 1955 | Lacks an alternate exon, results in a frameshift in the 3' coding region. |
C8orf58 has a relatively short 5’ region and a moderate 3’ region. Both the 5’ and 3’ regions contain stem loops.[9] There is one predicted miRNA binding site that found in the 3’UTR of C8orf58.[10]
Protein
[edit]C8orf58 protein Isoform 1 is 365 amino acids long. Isoform 2 and Isoform 3 are 357 and 300 amino acids respectively. There is a kozak consensus sequence present, which confirms it is a protein coding sequence.[11]
C8orf58 Isoform 1 has a molecular weight of 39.7 kDa and an isoelectric point of 8.29. It is proline and arginine rich and isoleucine, asparagine, phenylalanine, and tyrosine poor.[12]
The predicted secondary structure of the C8orf58 protein include multiple alpha helices and one beta strands.[12][13]
| Isoform | From mRNA Variant | Length (amino acids) | Molecular Weight (kDa) | Isoelectric Point |
|---|---|---|---|---|
| 1 | 1 | 365 | 39.7 | 8.30 |
| 2 | 2 | 357 | 38.6 | 8.30 |
| 3 | 3 | 300 | 32.0 | 5.82 |
Evolutionary history
[edit]It is part of the DUF4657 family, a family of proteins found in eukaryotes. Proteins in this family are typically between 305 and 370 amino acids in length.[14] The Domain of Unknown Function (DUF) of C8orf58 is located between amino acids 73 to 364.
Expression
[edit]According to the NCBI GEO profiles, C8orf58 is a narrowly expressed protein found in spleen, lung, thymus, prostate, and spinal cord tissue. It is constitutively expressed in these tissues.[15]
Post-translational modification
[edit]The bioinformatic tools on Expasy were used to determine potential post translational modification sites for the C8orf58 protein. There are two predicted phosphorylation sites and one predicted sumoylation site.[16]
Subcellular localization
[edit]According to PSORT II, C8orf58 is located in the nucleus. This is supported by the presence of a sumoylation site, which is involved in nucleic cytoplasmic transport.
Interacting proteins
[edit]Two proteins have been found to interact with protein C8orf58, CENPH and metG1, which were found using two hybrid assay and the two hybrid pooling approach respectively.[17] CENPH (Centromere Protein H) plays a critical role in centromere structure, kinetochore formation, and sister chromatid separation.[18] MetG1 (Methionine—tRNA ligase) is required for elongation of protein synthesis and the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation.[19]
Homology
[edit]An important paralog of this gene is ENSG00000248235.[20] Orthologs of the human gene C8orf58 are limited to vertebrates of the animal kingdom.
| Scientific Name | Common Name | NCBI Accession Number | Length (Amino Acids) | Date of Divergence (MYA) | Identity (%) | Similarity (%) |
|---|---|---|---|---|---|---|
| Homo sapiens | Human | NP_001013864.1 | 365 | - | - | - |
| Gorilla gorilla | Gorilla | XP_004046807.1 | 439 | 9.06 | 96 | 79.50 |
| Marmota marmota | Alpine Marmot | XP_015354979.1 | 369 | 90 | 68 | 75.7 |
| Oryctolagus cuniculus | European Rabbit | XP_008248092.1 | 371 | 90 | 66 | 72 |
| Nannospalax galili | Spalax | XP_008848689.1 | 362 | 90 | 65 | 74.7 |
| Ceratotherium simum simum | White Rhinoceros | XP_014652157.1 | 381 | 96 | 66 | 72.7 |
| Odobenus rosmarus divergens | Pacific walrus | XP_012418498.1 | 388 | 96 | 65 | 74.7 |
| Sus scrofa | Wild Boar | XP_005670472.1 | 382 | 96 | 65 | 73.3 |
| Hipposideros armiger | Great Roundleaf Bat | XP_019487131.1 | 387 | 96 | 62 | 71 |
| Eptesicus fuscus | Big Brown Bat | XP_008149784.1 | 377 | 96 | 62 | 70.1 |
| Loxodonta africana | African Bush Elephant | XP_003412428.1 | 372 | 105 | 71 | 77.2 |
| Orycteropus afer afer | Aardvark | XP_007949039.1 | 370 | 105 | 65 | 71.7 |
| Parus major | Great Tit | XP_015504136.1 | 320 | 312 | 32 | 35.6 |
| Anolis carolinensis | Carolina Anole | XP_008118367.1 | 453 | 312 | 28 | 38.9 |
References
[edit]- ^ a b c GRCh38: Ensembl release 89: ENSG00000241852 – Ensembl, May 2017
- ^ a b c GRCm38: Ensembl release 89: ENSMUSG00000044551 – Ensembl, May 2017
- ^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
- ^ "Entrez Gene: Chromosome 8 open reading frame 58". Retrieved 2017-11-22.
- ^ NCBI Nucleotide. Homo sapiens chromosome 8 open reading frame 58 (C8orf58), transcript variant 1, mRNA. [1]
- ^ GeneCard. C8orf58 Gene(Protein Coding) Chromosome 8 Open Reading Frame 58. [2]
- ^ NCBI Gene. C8orf58 chromosome 8 open reading frame 58 [Homo sapiens (human)]. [3]
- ^ RNA Folding Form
- ^ TargetScan Human
- ^ NCBI Protein. Uncharacterized protein C8orf58 isoform 1 [Homo sapiens].[4]
- ^ a b SDSC Biology Workbench
- ^ Chou-Fasman Secondary Structure Prediction Server
- ^ UniProtKB - Q8NAV2 (CH058_HUMAN). UniProt
- ^ NCBI GEO Profiles
- ^ Expasy Bioinformatics Resource Portal
- ^ IntAct Molecular Interaction Database
- ^ Centromere protein H
- ^ Methionine--tRNA ligase
- ^ GeneCard. 8orf58 Gene(Protein Coding) Chromosome 8 Open Reading Frame 58. [5].
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